ingredient
VECTOR
pET21b
INSERT
MTD
~20 amino acid
Name : Clostridium botulinum neurotoxin type A light chain
Lc
Amino acid: 447 amino acid Sequence : Clostridium botulinum type A Hall Lc
Tag
Hexa-histidine

OUR INGREDIENT

Structure of 'Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40'

‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ is developed as recombination of peptide called “MTD” (Macro-molecule Transmission Domain) that has function of skin and target cell permeation and, active protein of botulinum tox in called ‘Light Chain’. ‘MTD’ applied to our ingredient is originated and developed from botulinum tox in ‘Heavy Chain’, ,which performing a cell-penatration function of botulinum tox in.

STRUCTURE

MTD
Lc
His
469aa(53.6kDa)

COMPARISON OF BOTULINUM INGREDIENTS

toxin complex

Composition

  • Heavy chain : Nerve cell penetration
  • Light chain : Botulinum Efficacy Part
  • Hemagglutinin
  • Non-toxin Non-Hemagglutinin

Commercialization

  • 1980

Example

  • BO TOX (Allergan, US)
  • MEDITOXIN (Meditox, Korea)

Not permitted to use in Cosmetic Products

Molecular weight

  • 900KDa
pure toxin

Composition

  • Heavy chain: Nerve cell penetration
  • Light chain: Botulinum Efficacy Part

Commercialization

  • 1995

Example

  • XEO MIN (Merz, Germany)

Not permitted to use in Cosmetic Products

Molecular weight

  • 150KDa
our ingredient

Composition

  • Light Chain + MTD
  • Botulinum Efficacy Part
  • Marco-molecule Transmission Domain (Skin & Nerve cell penetration) = Recombination of the Protein

Commercialization

  • 2014

Example

  • BoLCA+ (BPMed cosmetic, Korea)
  • The First Botulinum Ingredient on ICID
  • BPMed Cosmetic has Exclusivity

Molecular weight

  • 50KDa

SKIN AND CELL PENETRATION TECHNOLOGY OF MTD

Platform technology to improve transdermal delivery of
‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’

  • 1st Action
    Improved penetration of functional materials into stratum corneum and other skin layers by MTD.
  • 2nd Action
    Maximized efficacy through the delivery of ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ into specific targets.
skin and cell penetration
cells

Modality of Intracellular delivery of MTD in ‘Methionyl
r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40′

Permeate in to cell by MTD technology shows very high Permeability and significantly less cell damage then nano liposome or common peptide ingredients(PTD).

MECHANISM ON NERVE CELL

Normal Neurotransmission Process

SNARE Complex Exocitosis Acetylcholine Diffusion
Binds to the Receptors on the Muscle

Penetrated in to dermis layer

Methionyl r-Clostridium Botulinum
Polypeptide-1 Hexapeptide-40

Nerve Cell

Approaching to the Cell

Direct Cell Membrane Translocation

SNAP 25

Moves to the SNAP25

Cleaves SNAP25 by Light Chain Part